Multiobjective Optimization of a Fed-Batch Bienzymatic Reactor for Mannitol Production

Enzymatic reactions can successfully replace complex chemical syntheses using milder reaction conditions and generating less waste. The developed model-based numerical analysis turned out to be a beneficial tool determine the optimal operating policies of multienzymatic reactors. As proved, for such cases, determination Fed-Batch Reactor (FBR) policy results in difficult multiobjective optimization problem. Exemplification is made bienzymatic reduction D-fructose mannitol by MDH (mannitol dehydrogenase) nicotinamide adenine dinucleotide (NADH) cofactor with situ continuous regeneration NADH at expense formate degradation presence FDH (formate dehydrogenase). For coupled system, engineering evaluations must account multiple competing (opposable) objectives. Among novelty elements: i) an optimally operated FBR tightly controlled variable feeding (of time stepwise type) during batch lead higher performance; ii) reported better performance compared single or cyclic BR, serial batch-to-batch reactors (SeqBR), when considering optimization; iii) concomitant substrate, enzymes, “time-arcs” option seldom approached literature but which proved here, leading consistent economic benefits.